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Figure 12 | Acta Neuropathologica Communications

Figure 12

From: Amyloid β and Tau Alzheimer’s disease related pathology is reduced by Toll-like receptor 9 stimulation

Figure 12

Assessment of CD45, CD206 and CD11b microgliosis. CpG ODN reduced overall hippocampal CD45 immunoreactivity in both treatment groups ( A - D ). Quantitative stereological analysis revealed 44% reduction (**p = 0.008) in CD45 reactive microglia burden in the 7–20 month study group and 52% reduction (**p = 0.0065) in the 11–18 month study group ( E , F ). Cortical CD45 immunoreactivity was minimal. Scale bar, 100 µm. Immunostaining with CD206 ( G - J ) followed by semiquantitative analysis demonstrated significant reduction in hippocampal CD206 positive cells in both age groups (7–20 months, *p = 0.04; 11–18 months, *p = 0.013) ( K , L ). Cortical CD206 was minimal. The degree of CD206 microgliosis was graded on a scale of 0–4. Scale bar, 100 µm. Representative immunostained images with CD11b microglia marker ( M - P ) and subsequent semiquantitative analysis of CD11b immunoreactivity revealed marked cortical (*p = 0.037) and hippocampal (*p = 0.015) reductions in CpG ODN-treated mice in the 11–18 month study group ( Q , R ). The degree of CD11b microgliosis was analyzed on a scale of 0–4. Scale bar (100 µm) in N corresponds to cortical images M, N. The scale bar in P corresponds to hippocampal images O, P.

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