Discrepancy in CCL2 and CCR2 expression in white versus grey matter hippocampal lesions of Multiple Sclerosis patients

Table 4 Primary and secondary antibodies used for double labeling

Antigen	Antigen retrieval	Species	Final dilution	Source primary antibody	Secondary antibody	Source secondary antibody	Conjugate	Source conjugate
Iba-1	Tris-EDTA	Goat	1:300	Abcam, ab5076	Alexa Fluor 488 coupled donkey anti goat IgG	Molecular Probes, A11055
GFAP	Citrate	Rabbit	1:4000	DAKO, Z0334	Alexa Fluor 594 coupled donkey anti rabbit IgG	Molecular Probes, A21207
CCL2	Citrate	Mouse	1:200	R&D Systems, MAB2791	Biotinylated goat anti mouse IgG	Jackson, 115-065-146	Alexa Fluor 488 coupled streptavidin	Molecular Probes, S11223
CCR2	Tris-EDTA	Rabbit	1:1000	Abcam, ab32144	Alexa Fluor 594 coupled donkey anti rabbit IgG	Molecular Probes, A21207
BM28	Tris-EDTA	Mouse	1:600	BD Transduction Laboratories, 610700	Biotinylated donkey anti mouse IgG	Jackson, 715-065-151	Alexa Fluor 488 coupled streptavidin	Molecular Probes, S11223

ISSN: 2051-5960