Figure 3

Semi-quantitative analysis of CCL2 positive cell numbers. CCL2 positive cells were only sporadically found within the hippocampal WM of control subjects (A) and MS patients without hippocampal lesions (D). The number of CCL2 positive cells was slightly increased within the WM centre of active (B) and inactive (C) lesions and borders (F). A significant increase in the number of CCL2 positive cells was found within the WM border of active lesions (E). CCL2 positive cells were barely detected within the hippocampal GM of control subjects (G), MS patients without hippocampal lesions (J), the lesion centre (H) and border (K) of active lesions and only sporadically within the lesion centre (I) and border (L) of inactive lesions. Semi-quantification of CCL2 positive cells (M). Scale bar (A-L) = 60 µm; + p < 0.05 versus cell number in WM of non-demyelinated hippocampi of MS patients and in hippocampal WM of control subjects; # p < 0.05.