Figure 8
From: Pathological alpha-synuclein propagates through neural networks
Sequential transmission of insoluble αsyn. (A) Schematic diagrams of transmission experiments. Sarkosyl-insoluble fractions were prepared from WT mouse brains injected with recombinant αsyn fibrils at 9 months post-injection and intracerebrally injected into 4-month-old WT mice. At 3 months after injection, transmission of αsyn pathology was analyzed by IHC. (B) Immunoblot of sarkosyl-insoluble fractions from WT mice at 9 months post-injection with #64 antibody and anti-msyn antibody. (C) Psyn pathology was observed mainly in Str, amygdala and SN with 1175 antibody. Scale bar represents 50 μm. (D) Distribution of psyn pathology in WT mouse brains injected with sarkosyl-insoluble fractions. Blue-dashed box and red dots indicate the injection site (Str) and psyn pathology, respectively.