Excessive p62 accumulation in the VPM/VPL of PGRN-deficient mice with aging. A, p62-immunostained images with Nissl counterstaining in the VPM/VPL of 10- and 90-week-old WT and KO mice (scale bar = 100 μm). B, C, p62-positive area ( B ) and p62 gene expression ( C ) in the VPM/VPL of 90-week-old WT and KO mice. The data are presented as the change relative to the level of WT mice. Values are the mean ± SEM ( B: n = 5 for each group; C: n = 6 for WT, n = 5 for KO, *P < 0.05, unpaired t-test). D, High-power p62-immunostained image with Nissl counterstaining (scale bar = 20 μm). E, Double-stained images of p62 (green) with MAP2 (red), GFAP (red), MBP (red), and CD68 (red) in the VPM/VPL of 90-week-old KO mice (scale bar = 10 μm). F, The triple-stained image of p62 (green), ubiquitin (red) and Hoechst33258 (blue) in the VPM/VPL of 90-week-old KO mice (scale bar = 10 μm).