Figure 3

Increased lysosomal biogenesis in the cerebral cortex and VPM/VPL of aged PGRN-deficient mice. A, Lamp1-immunostained images of the layer V of the cerebral cortex (upper images) and VPM/VPL (lower images) of 10- and 90-week-old wild-type (WT) and PGRN-deficient (KO) mice (scale bar = 20 μm). B, C, Lamp1-positive area in the layer V of the cerebral cortex ( B ) and VPM/VPL ( C ) of 10- and 90-week-old WT and KO mice. The data are presented as the change relative to the level in 10-week-old WT mice. Values are the mean ± SEM (n = 5 for each group). Values having a different superscript are significantly different (P < 0.05, Tukey-Kramer test). D, Gene expressions of Ctsd, Atp6v0d2, and Tfeb in 90-week-old WT and KO mice. The data are presented as the change relative to the level in WT mice. Values are the mean ± SEM (n = 6 for WT; n = 5 for KO, *P < 0.05, unpaired t-test).