Characterisation of myelin, oligodendroglia, microglia/macrophages and axons following demyelinating lesion produced by LPC application in the adult zebrafish optic nerve. (A-D) There is (A) a significant reduction in Claudin k (myelin) immunoreactivity (arrows, p < 0.001), (B) fewer olig2:dsred positive oligodendroglia in the lesion site at 4 to 14dpl (arrows, p < 0.05), (C) transiently increased numbers of 4C4 immunoreactive microglia/macrophages at 4 to 8dpl (arrows, p < 0.001) and (D) no change in LINC + axon pixel intensity. Pixel intensities in A and D are presented as a ratio between lesioned nerve and unlesioned contralateral control. Dashed lines outline optic nerve. A minimum of n = 5 fish were used for each time-point. Mean ± SEM. All ANOVA and Tukey’s post test. Scale bar: A-D = 100 μm.