Fig. 1

Co-immunofluorescent staining against p-tau217 and p-tau 181, p-tau 231, p-tau 202/205, p-tau 202 and p-tau 369/404. Images represent immunostaining of CA1 of an AD patient. Images in (A–O) show that p-tau217 (in red) is found in tangles, neuropil threads and in vesicle-like structures. Images in (A, D, G, J and M) show associations between tangles positive for p-tau217 (red) and p-tau181 (green in A), p-tau 231 (green in D) and p-tau 202/205 (green in G), p-tau 202 (green in J) and p-tau 369/404 (green in M). Images in (B, E, H, K and N) show that most neuropil threads positive for p-tau217 (red) also are positive for p-tau 181 (green in B), p-tau 231 (green in E) and p-tau 202/205 (green in H), p-tau 202 (green in K) and p-tau 369/404 (green in N) (indicated with longer arrow). Vesicle-like structures were clearly visualized in the p-tau217 staining (red) (arrows in in C, F, I, L and O), but most of them were not associated with the other five p-tau variants (indicated with short arrow) and only a few vesicles were also positive for p-tau 181 (green in C), p-tau 231 (green in F) and p-tau 202/205 (green in G) and p-tau 369/404 (green in M) (indicated with long arrow). Scalebar = 15 µm