Fig. 4
Activated/phagocytic microglia arise during demyelination. a Workflow of the experiment. b UMAP depicting 12,885 cells. Colors depict unsupervised clustering. c Bar plot depicting cluster distribution per group with Chi-squared statistical analysis. d UMAPs depicting gene expression levels of Mki67, Phlda3, Lpl, Ifit3 and Tmem119. Immunofluorescent staining of caudate putamen region. Hoechst (blue; nuclei), IBA1 (green; macrophages), Mki67 (magenta; Mm6). Arrowheads indicate IBA1posKI67pos cells. e Representative images of IBA1 immunohistochemistry in the cortex, corpus callosum and caudate putamen. f Quantification of immunohistochemistry of 2–3 mice per group. *: p < 0.05; **: p < 0.01; ***: p < 0.001. Abbreviations: C = control; D3 = 3-weeks demyelination; D5 = 5-weeks demyelination; R = remyelination