Figure 5
From: Loss of the neuroprotective factor Sphingosine 1-phosphate early in Alzheimer’s disease pathogenesis
S1P phosphatase activity increases in temporal GM of AD brains. Total S1P phosphatase activity in hippocampus (A) and temporal GM (B) extracts of control (n = 9), Braak stage I/II (n = 8), Braak III/IV (n = 7), and Braak V/VI (n = 10) brains. Overall association between S1P phosphatase activity activity and Braak stage was significant (P < 0.0001 by one-way ANOVA) for temporal GM but not hippocampus (P = 0.063). Sgpp1 (C) and Sgpp2 (D) protein levels in the temporal GM tissue extracts were determined by western blotting and normalised to housekeeping gene β-actin. (E) Example western blots for Sgpp1 and Sgpp2 protein. Braak stage is indicated for each sample.