Proposed criteria for the classification of mMCD II in epilepsy. (A) This may be used as practical guidelines for neuropathologists to diagnose mMCD II in the temporal cortical resections from patients with mTLE and hippocampal sclerosis. Values are based on the present study which have used cortical resections (inclusive of the superior, middle, and inferior temporal gyrus that were 1–1.5 cm from pole), and immunohistochemistry using anti-NeuN (Millipore MAB377, 1:100, overnight). (B) In this study, WMN density values obtained using WSA manual, whereby the area of interest was outlined a set distance (0.5 mm) from the grey-white matter boundary, was regarded as the gold standard in the evaluation of neuronal counts. A graphical representation of our proposed numerical thresholds is presented here. Each point represents one case. WSA = whole slide imaging with automated analysis, control max = the highest WMN density value from controls, control mean = mean WMN density of controls, mMCD = mild Malformation of Cortical Development, SA = semi-automated analysis, sd = standard deviation, WMN = white matter neurons.