Iba1 positive microglia are not altered following steroid treatment. WT Iba1 immunoreactivity was very low in the cranial motor nuclei (A, E, I), and in the spinal cord (M). SOD1
G93A mice treated with saline (B, F, J, N), free MP (C, G, K, O) and 2B3-201 (D, H, L, P) showed much stronger staining for Iba1, both in the three brainstem nuclei and the spinal cord. No difference was found in immunoreactivity between SOD1
G93A treatment groups. Panels Q, S, U and W show quantification of Iba1 staining intensity in the brainstem motor nuclei and spinal cord, and panels R, T, V and X show the density of Iba1+ microglia in these same regions, showing increased expression in all SOD1G93A treatment groups compared with WT mice, but no group differences among treatments. Scale bar applies to all images. * p<0.05; ** p<0.01; *** p<0.001.