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Figure 1 | Acta Neuropathologica Communications

Figure 1

From: Tau pathology does not affect experience-driven single-neuron and network-wide Arc/Arg3.1 responses

Figure 1

In vivo quantification of Arc::dVenus reporter in the visual cortex of rTg4510 mice. (a) Experiment outline. Structural visual stimulation paradigm described previously in [10] was followed by cranial window implantation over the medial extrastriate visual cortex and imaging on a multiphoton microscope. After the imaging, the brains were collected and processed for immunohistochemical analysis. (b) Sample maximum intensity projections of in vivo-acquired multiphoton z-stacks from the layer II/III of the medial extrastriate visual cortex, showing Arc::dVenus reporter (shown in green) expression induced by the visual stimulation. Scale bar = 100 μm. (c) Frequency distribution histograms of activity-induced Arc::dVenus fluorescence in individual visual cortex neurons of rTg4510 mice (n = 8 mice, 2212 neurons) and control littermates (n = 5 mice, 1043 neurons). AU, arbitrary units. n.s., not significant, P = 0.27. (d) Sample single-section in vivo multichannel micrographs of the same visual cortex site from an rTg4510 mouse before (left) and after (right) the intravenous delivery of a tangle-binding dye methoxy-X04 (red). Fluorescent angiogram (blue) was used as a landmark pattern during the image analysis. Arrowheads points to a dVenus-positive neuron with an NFT and the inset shows a close-up view of this neuron. Scale bar = 100 μm. Inset size = 20 × 20 μm. (e) Frequency distribution histograms of activity-induced Arc::dVenus fluorescence in rTg4510 neurons without tangles (n = 4 mice, 720 neurons) and with tangles (n = 4 mice, 101 neurons). AU, arbitrary units. n.s., not significant, P = 0.083.

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