Figure 4
Quantification of Aβ 40 and Aβ 42 levels in cortex and hippocampus by sandwich ELISA. TBS-soluble a) Aβ40 and b) Aβ42 levels in cortex + hippocampus homogenates from non-transgenic (-/-), heterozygous (+/-) and homozygous transgenic (+/+) rats at different ages (3 months, 7 months and 13–15 months) were quantified with specific G2-10/W0-2 and G2-13/W0-2 sandwich ELISAs, respectively. TBS-insoluble pellets were extracted with 70% formic acid (FA) and after neutralization, c) Aβ40 and d) Aβ42 levels in FA fractions were assayed with G2-10/W0-2 and G2-13/W0-2 ELISAs, respectively. Values were normalized to total protein concentration and expressed as means ± SEM. One-way ANOVA, followed by Dunnett’s post-hoc test. *P < 0.01, **P < 0.001, ***P < 0.0001 (across the same time point), # P < 0.01, ## P < 0.001, ### P < 0.0001 (across ages).