Intensity of 4-HNE adduct staining. Panels A and B are the graphical representation of the fluorescence intensity recorded for 4-HNE adduct staining vs. the total 4-HNE staining neurite area for both 3- and 6-month STZ-diabetic and age-matched control rat neuronal cultures. An asterisk (*) indicates statistically significant data. Error bars indicate the mean ± the S.E.M. At 3 months (panel A), there was no statistical significance in 4-HNE adduct staining between diabetic and control neurons (p = 0.5755). At 6 months (Panel B), there was a significant difference observed, with the diabetic neurons displaying a 4-HNE adduct staining intensity per total 4-HNE staining area that was 2.22X that seen in the controls (p = <0.0001).