Morphological assessment of neuronal dystrophy. Panels A and C represent data collected from the 3-month neuronal cultures. As panel A indicates, at 3 months, there were significantly higher proportions of mildly (34.9% vs. 21.1%, p = 0.0244) and highly dystrophy (53.2% vs. 29.5%, p = 0.0004) neurons in the diabetic neurons vs. the control neurons. Panel C indicates there was a significantly higher proportion of any dystrophy (mild or high) in the diabetic vs. the control neurons (88.1% vs. 50.5%, p = <0.0001). Panels B and D represent data collected from 6-month neuronal cultures. At 6 months, there was a significantly higher proportion of mild dystrophy in diabetic neurons vs. control (39.4% vs. 27.4%, p = 0.008), but no statistical significance to the proportions of neurons that were highly dystrophic. Additionally, as seen in Panel D, there was a significantly higher proportion of any dystrophy (mild or high) in the diabetic neurons vs. the controls (74.3% vs. 62.2%, p = 0.0448). Error bars indicate the mean ± the S.E.M.