Figure 3

Immunofluorescence of neuronal cultures. Images were captured on a Carl Zeiss LSM510 inverted confocal microscope with a 40x objective. Panels A (age-matched control) and B (STZ-diabetic) are images collected from the 3-month rat cultures of PGP-9.5 and 4-HNE co-stained neurons, while panels C (age-matched control) and D (STZ-diabetic) are images of the PGP-9.5 and 4-HNE co-stained neurons from the 6-month rat cultures. The dystrophic feature of neurite swelling (yellow arrows) is indicated in panels B and D, and examples of neurite branching (blue arrows) can be seen in panels A, B and D.