Figure 3

H3K9me3 immunoreactivity is no different in DIPG, adult GBM, and pediatric GBM. H-scores for Histone 3 Lysine 9 trimethylation (H3K9me3) immunoreactivity in normal brain (Control), adult glioblastoma (aGBM), pediatric glioblastoma (pGBM), and diffuse intrinsic pontine glioma (DIPG) tissue (p < 0.0001, Kruskal-Wallis ANOVA Test) (a). H3K9me3 immunoreactivity was relatively preserved in DIPG and not significantly different from extrapontine GBM (adult GBM p > 0.05, pediatric GBM p > 0.05, Kruskal-Wallis test of multiple comparisons). A significant difference was noted between tumor groups and normal brain (p < 0.05, Kruskal-Wallis test of multiple comparisons). Number of samples per group and median H-scores are specified in b.