Figure 2

H3K27me3 is decreased in DIPG. H-scores for Histone 3 Lysine 27 trimethylation (H3K27me3) immunoreactivity in normal brain (Control), adult glioblastoma (aGBM), pediatric glioblastoma (pGBM), and diffuse intrinsic pontine glioma (DIPG) tissue (p < 0.0001, Kruskal-Wallis ANOVA Test) (a). H3K27me3 median H-score was significantly lower for DIPGs compared to pediatric GBM (p < 0.01, Mann–Whitney Test), adult GBM (p < 0.0001), and normal brain (p < 0.0001) tissue. A significant difference was also noted in immunoreactivity between control and adult GBM (p < 0.0001) and also control and pediatric GBM (p < 0.0001). No difference was noted between adult and pediatric GBM immunoreactivity for H3K27me3 (p > 0.05). Number of samples per group and median H-scores are specified in b.