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Table 5 Analysis of the IHC/PCR and PCR/Sanger sequencing discordant cases

From: A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma

Case IHC IDH1/2 PCR Sanger sequencing Pyrosequencing LNA-sequencing Result Conclusion
# 1 (commercial) Pos WT WT WT WT WT IHC False-Pos
# 2 Pos R132H WT - - R132H Low Mutant Allele% (15%)
# 3 Pos R132H WT - - R132H Low Mutant Allele% (10%)
# 4 (commercial) Neg R132C WT R132C - R132C Low Mutant Allele% (14%)
# 5 (commercial) Neg R132 WT WT WT WT PCR False-Pos*
# 6 Neg R172 WT WT WT WT PCR False-Pos*
  1. IHC: Immunohistochemistry; PCR: Polymerase chain reaction; WT: Wild-type; Pos: Positive; Neg: Negative; LOD: limit of detection.
  2. *PCR test result close to LOD value.