Sensitivity of the IDH1/2 PCR assay for the 12 detected and 3 identified IDH1/2 mutations. The sensitivity defined as the lowest amount of mutant DNA in a background of wild-type DNA was indicated for each of the 12 IDH1/2 mutations detected with the PCR IDH1/2 kit. The cut-off values were derived from a total of 2250 ΔCt measurements. Sensitivity varied across mutations from 0.6% to 15% with a mean of 3.3% (red dotted line). Limit of Detection was <5% for 11/12 mutations and ≤3% for 9 of them.