Figure 2

Tau APFs are present in DLB and PSP brain tissue. The age, gender, post-mortem interval (PMI), and sampled brain regions in each group is summarized (a). APFs were detected in DLB cortex (top panel) and PSP pons (bottom panel) by light field microscopy after immunolabeling with αAPF (b). After immunoprecipitation using αAPF, tau annular structures were detected in DLB and PSP cases but not in age-matched control brain tissue (top, cortex; bottom, pons) by dot-blot analysis using Tau-5 antibody (c). Tau amyloid pores isolated from DLB (top) and PSP (bottom) brain tissue were visualized by AFM (d). Scale bar for HRP staining, 10 μm. Scale bar for AFM images, 30 nm. White arrowheads indicate tau APFs.