Figure 3

(a-o) As noted in the margins of the figure, tissue sections from mice overexpressing WT, G93A, G37R, and L126Z hSOD1, or nontransgenic mice for controls, were stained with C4F6 following either no antigen retrieval, formic acid and 6 M guanidine hydorchloride (FA & GdnHCL), or steaming in citrate buffer. All tissues from mice expressing mutant hSOD1 (G93A, G37R, and L126Z) were harvested from paralyzed mice. The WT hSOD1 tissue was from mice ~18 months of age. Arrows in panels d and f highlight staining around the vacuolar structures commonly present in G93A mice. Scale bar 100 μm.