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Figure 1 | Acta Neuropathologica Communications

Figure 1

From: A new animal model of spontaneous autoimmune peripheral polyneuropathy: implications for Guillain-Barré syndrome

Figure 1

Transgene-derived B7.2 constitutive expression primed macrophages and microglia in the nervous tissues. The number of CD11b+ CD45+ macrophages in the sciatic nerves and microglia in the spinal cord, and the expression of B7.2 among these targeted cells was quantified using fluorescent-activated cell sorting (FACS) analysis. The histogram represents the FACS analysis obtained from 3 animals per group. Percentage of either macrophages (A) or microglia (C) expressing B7.2 is increased in L31/CD4-/- mice, compared with wild type animals. The absolute number of macrophages in the nerves and microglia in the spinal cord is also higher in L31/CD4-/- mice than that of wild type mice (A, C) (*: p < 0.05, ***: p < 0.001). Representative examples of immunohistochemistry analysis of macrophages in the sciatic nerves and microglia in the spinal cords were depicted in (B) and (D). Note that in L31 /CD4-/- mice, many Iba-1+ macrophages and microglia were colocalized with B7.2 which was almost undetectable in wild type mice. Scale bars: B, 50 μm; D, 500 μm.

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