Figure 4

Early NMO pathology and NMO pathology produce from NMO patient serum. A. AQP4, GFAP, MBP and albumin immunofluorescence in rat brain at 1 day after intraperitoneal injection of NMO-IgG and intracerebral needle injury. B. Staining for activated complement (C5b-9), leukocytes (CD45), neutrophils (Ly6-G) and macrophages (CD163) at 1 day. C. Rats were administered 75 mg of purified IgG from NMO patient serum (or non-NMO control serum) as in Figure 2A (top). In vitro cytotoxicity of rat serum obtained at 8 h measured in AQP4-expressing CHO cells by Alamar blue assay as in Figure 2A (bottom) (S.E., n = 3). D. (Left) AQP4, GFAP and MBP immunofluorescence at 5 days in rats administered purified IgG from NMO patients (or control non-NMO IgG). (right) Summary the areas loss of AQP4, GFAP and MBP (S.E., 4 rats).