Inflammation and blood–brain barrier disruption in the NMO rat model. A. MBP and neurofilament (NF) immunofluorescence at 5 days in rats treated as in Figure 2. White line demarcates the lesion. B. Albumin immunofluorescence shown with quantification of immunopositive area (S.E., n = 3). C. Iba1, iNOS (M1-macrophage marker) and arginase-1 (M2-macrophage marker) immunofluorescence. D. (left) Hematoxylin and eosin (H&E) staining, and immunofluorescence for activated complement (C5b-9), leukocytes (CD45), neutrophils (Ly6-G) and macrophages (CD163) in brain at 5 days. (right) Summary of the number of infiltrating cells per 0.01 mm2 (S.E., 3 rats).