Figure 5

GAN-linked Gigaxonins exhibit shorter half-lives. A Representative autoradiograms and immunoblots of the pulse chase assay for wild type, mutant Gigaxonins, and the BTB (N-ter) and KELCH (C-ter) domains. At different times (2, 4, 6, 9, 24 h) after the beginning of the chase (0 h), Gigaxonin was immunoprecipitated and processed for autoradiography (top panel). The signals were normalized to the tubulin immunoblotting of the supernatent fractions of the IPs and plotted at 100% for the time point 0 h. B Half-lives of Gigaxonins. Each of the 3–4 experiments realized per construction is plotted on the graph, to define the corresponding curve.