Figure 6From: Optimization of electron microscopy for human brains with long-term fixation and fixed-frozen sections Examples of potential preparations that can be applied to fixed-frozen sections adjacent to the STG section used for EM on case 3. (A) Nissl (thionin) cell body stain. (B) Higher magnification of (A) showing layers of STG cortex. (C) Example of pyramidal neuron from (B) at 100× magnification used for stereological estimation of neuron number. (D) Iba-1 stained section for microglia. (E) Parvalbumin stained section through STG of case 3. Additional protocols available upon request. Back to article page