Figure 5

Quantitative analysis of myelinated axons from EM samples of case 1a, b, 2 and 3. (A) Histogram of myelinated axon density (mean number of myelinated axons per square micron (μm²). On average, 900 myelinated axons were counted in ~6000 μm² area for each case. Case 1a fixed, case 1b fixed, and case 1 frozen do not differ in myelinated axon density. Case 2 fixed and frozen also do not differ in myelinated axon density. Case 2 and 3 have higher axon density relative to case 1 (p < 0.05), regardless of preparation method. (B) Histogram showing percentage distribution of myelinated axons size. Case 1a and case 1b are grouped as case 1. Graph displays similar size distribution pattern in case 1, 2 and 3. Approximately 70% of myelinated axons have diameters ranging from 0.25 μm to 0.75 μm. (C) g-ratio (average 0.63) histogram demonstrating no significant differences between cases. (D) Scatterplot showing positive correlation of g-ratio and myelinated axon diameter (r = .38, p < .01).