Figure 3
From: Generation of iPSC lines from archived non-cryoprotected biobanked dura mater
Characterization of additional dura-derived iPSCs. All scale bars are 100 μm and representative clones are shown. ASC19D-ALS (clone 1) is from a sporadic ALS case. ASC27D-HD (clone 2) is from a Huntington’s disease case. ASC30D-PD (clone 1) is from a sporadic Parkinson’s disease case. (A-C) Outgrowth with fibroblast morphology from dura mater. (D-L) Immunostaining for pluripotency markers as indicated. AP stands for alkaline-phosphatase. DNA is in blue. (M-R) Undirected EBs were cryosectioned and immunostained for the three developmental germ layers: endoderm (AFP), mesoderm (SMA), and ectoderm (Tuj1). (S-U) iPSC-derived neurons (Tuj1) and neural progenitors (Pax6) after 14 days of directed neuronal differentiation. DNA is in blue.