Figure 7

Reduced endosome levels after dynein-dynactin inhibition. A-D) Primary hippocampal neurons expressing the dynactin subunit p50 (GFP-p50), a dominant-negative dynactin construct (GFP-p150-cc1) or GFP-BICD2-N, show reduced EEA1 labelling and a lower EEA1 particle density compared to GFP transfected and untransfected neurons (A). Inset shows enlargement of soma. Bar: 10 μm. E) Bar graph of the relative particle density. ***, P < 0.001 compared to GFP group, Tukey’s multiple comparison test following one-way ANOVA (P < 0.001).