Figure 6

Reduced endosome levels in motor neurons with Golgi fragmentation. A, B) Confocal images of staining of the endosomal proteins EEA1 (A) and GRASP1 (B) in lumbar spinal cord sections of a BN1 transgenic mouse. Note reduced EEA1 and GRASP1 labeling in GFP-BICD2-N positive neurons. C) Analysis of fluorescent intensities of GFP-BICD2-N positive versus negative motor neurons showed reduced mean intensities in GFP-BICD2-N positive motor neurons (P < 0.05, unpaired Student's t-test negative (-) versus positive (+) neurons). Note that all values were collected from non-transgenic (n = 2 mice) and BN1 (n = 4) lumbar spinal cord specimen embedded in a single gelatin block and processed in the same immunorun (see Material and methods). D-F) Quantitation (D) and representative images (E, F) of GRASP1-labeling in motor neurons with normal and fragmented Golgi showing reduced GRASP1 labeling in motor neurons with fragmented Golgi (P < 0.05, unpaired Student's t-test, normal versus fragmented Golgi). G, H) Confocal images of EEA1-labeling in motor neurons with normal and fragmented Golgi showing reduced EEA1 labeling in motor neurons with fragmented Golgi. Bars, 10 μm.