Figure 4

Golgi fragmentation in SOD1-ALS mice is not downstream of apoptotic pathways. A, B) Confocal images of GM130 and ATF3 labeling in spinal motor neurons of a 90 day old fast SOD1-G93A mouse (G1 line) crossed into mouse deficient for BAK in all cells and BAX specifically in neurons (DKO) resulting in deficiency of the mitochondrial apoptotic pathways. Note, motor neurons with normal (A) and fragmented (B) Golgi apparatus and ATF3 expression in motor neurons with fragmented Golgi. C) G1-DKO showed a lower proportion Golgi fragmented motor neurons but this difference was not significant (unpaired Student’s t-test); values are means ± SE (n = 3 mice per group). D) Confocal image of p115 and GM130 (N-terminus) double labeling in motor neurons in 25 week old G1del mouse showing that motor neurons with fragmented Golgi (insert) show the same relative intensity of p115 staining. Bar: 10 μm.