Figure 3

CTB tracing and Golgi fragmentation in motor neurons expressing ATF3 or contacted by phagocytosing microglia. A) Confocal image showing a CTB-positive motor neuron with fragmented Golgi and ATF3-positive nucleus (arrow). B) Confocal image of motor neurons with fragmented Golgi that are ATF3-negative (arrows) and a motor neuron with a normal Golgi apparatus that is ATF3-positive. C, D) Maximal projections of confocal stacks showing CTB-positive motor neurons contacted by Mac-2 positive microglial cells. Bars: 20 μm (A-C).