Figure 3

TDP-43 colocalizes with p65 in the neuronal cells from the temporal cortex of individuals with MCI-p. a-d Sections from the temporal cortex of MCI-p (a, Subjects 8 and 15), MCI without the interaction of TDP-43 with p65 in coimmunoprecipitation experiments (Figure 1B, C; MCI-n, b, Subjects 6 and 12), AD (c, Subject 2) or NCI (d, Subjects 7 and 21) showing the interaction of TDP-43 with p65 (Figure 1B,C; AD-p or NCI-p) were incubated with anti-TDP-43 and anti-p65 antibodies and subsequently with corresponding Alexa 488 and 633 antibodies, and imaged by confocal laser microscopy. Autofluorescence was detected using 575–630 nm bandpass emission filter. Arrows indicate the nuclear TDP-43 positive cells colocalized with p65 (a). Arrowheads indicate the nuclear TDP-43 positive cells without colocalization with p65 (a-d). Scale bars, 50 μm.