Figure 2

TDP-43 colocalizes with p65 in the nucleus of neurons of the temporal cortex of individuals with MCI showing the interaction of TDP-43 with p65 in coimmunoprecipitation experiments (Figure 1 B, C; MCI-p). a, b A section from the temporal cortex of MCI-p (Subject 15) were incubated with anti-p65 and anti-TDP-43 (a) or anti-NeuN (b) antibodies and subsequently with corresponding Alexa 488 and 633 antibodies (Molecular Probes), and imaged by confocal laser microscopy. The nuclei were counterstained with Dapi. Autofluorescence was detected using 575–630 nm bandpass emission filter. Arrows indicate colocalization of Dapi, p65 and TDP-43 (a) or NeuN (b). Scale bars, 50 μm.