Figure 1

Full-length TDP-43 interacts with p65 NF-κB in the temporal cortex of four individuals with mild cognitive impairment (MCI). a The presence of phosphorylated p65 NF-κB at ser536 and accumulation of p65 and full-length of TDP-43 in TBS-soluble fraction from the temporal cortex of individuals with MCI. Protein extracts from the temporal cortex of individuals with no obvious cognitive impairment (NCI; N, n = 12), MCI (M, n = 12) or Alzheimer’s disease (AD; A, n = 12) were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. Actin was used as a loading control. b, c Interaction of TDP-43 with p65 in TBS-soluble fraction from the temporal cortex of four individuals with MCI (Subjects 8, 15, 17 and 23), one individual with AD (Subject 2) and four individuals with NCI (Subjects 7, 14, 21 and 29). Protein extracts from the temporal cortex of individuals with NCI, MCI or AD were used for immunoprecipitation (IP) with anti-p65 polyclonal antibody (b) or anti-TDP-43 polyclonal antibody (c). Immunoprecipitates were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. Number of Subjects 8, 15, 17 and 23 is written in red color.