Figure 1

APLP2-KO CA1 neurons show a WT-like morphology. (a) Schematic representation of morphometric Sholl analysis. The number of intersections between dendrites and concentric spheres centered on the soma was determined at various distances from soma (30 μm increments). Sholl analysis of basal (b) and apical dendrites (c) of CA1 pyramidal neurons from APLP2-KO and WT mice revealed no significant alterations in dendritic morphology (Repeated measures ANOVA with Bonferroni’s multiple comparison test, n.s.). Neuron reconstruction and analysis were done using the Neurolucida and Neuroexplorer software (Microbrightfield). (d-f) Neither significant alterations in the number of primary basal dendrites (WT: 5.06 ± 0.37 versus APLP2-KO: 5.60 ± 0.43; Student’s t-test p > 0.05) (d) nor changes in the total dendritic complexity (e) or total dendritic length (f) were observed (Student´s t-test, n.s.). WT: n = 32 neurons/ 6 mice, APLP2-KO: n = 25 neurons/ 5 mice. Values represent mean ± SEM.