Brain lesions caused by MOG-IgG and AQP4-IgG. Mice received IgGCON + Chu (purple), IgGMOG + Chu (green) or IgGAQP4 + Chu (blue), were killed at 24 hours (d1), seven days (d7) or two weeks (d14) and coronal brain sections were cut through the injection site. A. H + E staining. Ed, edema; L, lumen; leuko, leukocytes; RG, reactive glia; wm, white matter. B. (Top) LFB staining. Red line, loss of LFB staining. (Bottom) % Loss of LFB stain (area without LFB/ipsilateral hemispheric area) vs. days since injection. C. (Top) CD45 immunostain. Each boxed area in d7 top is shown magnified below. Arrows, CD45+ cells; L, vessel lumen; mϕ, macrophages. (Bottom) % CD45+ area (CD45+ area/ipsilateral hemispheric area) vs. days since injection. Mean ± SEM, 5 mice per group. P < 0.01**, 0.001*** (compared with each of the other two groups). Bar 50 μm (A), 1 mm (B, C d7 top), 20 μm (C d7 bottom).