Figure 3
From: Novel monoclonal antibodies to normal and pathologically altered human TDP-43 proteins
IHC and IB properties of new N- and C-terminal specific anti-TDP-43 MAbs. N-terminal specific MAb 5031 reacts with spinal cord ALS TDP-43 pathology (a) with minimal detection of FTLD cortical TDP-43 pathology (arrow, c), while MAb 5056 shows robust reactivity for both spinal cord (b) and cortical TDP-43 inclusions (arrows, d). Consistent with previous studies [13], immunoblot analysis shows that MAb 5031 detects full-length but not the CTF of TDP-43 (e), while C-terminal specific MAb 5056 recognizes both (f). Scale bar = 100 μm.