Representative IHC staining patterns and immunoblot profiles of selected MAbs that recognize human- or human- and mouse-TDP-43. MAb 5195 detects normal nuclear TDP-43 in (a) human and (b) mouse hippocampus dentate gyrus. In contrast, MAb 5095 detects (c) human but not (d) mouse TDP-43, which is representative of 7 MAbs that preferentially recognize human TDP-43. Scale bar = 50 μm. (e) All the MAbs here show strong immunoreactivity with a 43 kDa band from human cell lysates, but 7 MAbs preferentially recognize human TDP-43 with little or no detection of mouse TDP-43. MAb clone numbers are shown above each immunoblot, and MAb 5195 is representative of 8 MAbs that detect human and mouse TDP-43 with equal affinity. H – human, M – mouse.