Figure 8

Effects of SRTAW04 on inflammation in the spinal cord. (a) Spinal cord sections (n = 5 per group) from 7 day post infected MHV-A59 and MHV-A59 infected group treated with 100 mg/kg SRTAW04 daily stained with H&E were scored using a relative inflammation scale. There is a significant increase (*p < 0.05) in the inflammation score in the MHV-A59 treated mice compared to controls. No significant difference in the inflammation score is observed between the MHV-A59 infected group and MHV-A59 infected mice treated with SRTAW04. By day 30 post-innoculation, limited inflammation is observed by H&E staining in spinal cord, and inflammation that does occur consists almost exclusively of macrophages/microglia, thus sections (n = 4-5 per group) were immunostained for Iba1 to further assess relative levels of inflammation. A representative spinal cord section is shown from (b) control, (c) RSMHV2 infected, (d) RSA59 infected, (e) RSA59 infected + SRTAW04 treatment and (f) RSA59 infected + SRTAW04 + EX527 treatment mice. Boxes in low magnification sections (left column) indicate area shown at high magnification (right column). Increased foci of Iba1 positive cells are present in RSA59 infected mice with or without SRTAW04 treatment compared to control and RSMHV2 mice. Scale bars 50 μm for b-f.