MHV-A59 infection induces RGC loss. (a) Inoculation with demyelinating strains MHV-A59 (n = 12), and its isogenic recombinant strain RSA59 (n = 10), lead to significantly decreased (***p < 0.001) RGC numbers compared to non-infected control mice (n = 10), and mice inoculated with the non-demyelinating control strain MHV2 (n = 16) (@@@p < 0.001). Mice infected with MHV2 did not show RGC loss compared to non-infected controls. Fluorescently labeled RGCs are shown from a representative field in (b) non-infected control and (e) MHV2 infected retina, whereas fewer RGCs are seen in corresponding areas of retina in MHV-A59 (c) or RSA59 infected mice (d). Scale bars 10 μm for b-e.