Detection of disease-associated α -synuclein ( α S
) in different neuroanatomical regions of M83 mice after stereotactic inoculations in the hippocampus (A-C) or cerebellum (D-F) A, D. Detection of αS on sagittal brain sections by immunohistochemistry using PSer129 antibody. (Scale bar, 1 mm. Insets are high magnifications of different areas). Arrows indicate the approximate sites of the stereotactic inoculations. B, E. Detection of insoluble pSer129 αS by Western blot in selected neuro-anatomical regions. β-actin was used as loading control. C, F. ELISA results with LB509 and PSer129 antibody in selected neuro-anatomical regions. Hi: hippocampus (Hi), C: contro-lateral side, I: inoculated side, Cb: cerebellum, BS: brainstem, SC: spinal cord.