Figure 4
Detection of disease-associated α -synuclein ( α S D ) in different neuro-anatomical regions of M83 mice by ELISA and Western blot. The following neuro-anatomical regions were sampled from sick M83 mice at first passage: OB: olfactive bulb, Cx: cerebral cortex, Hi: hippocampus, St: striatum, Me: mesencephalon, Cb: cerebellum, BS: brain stem, SC: spinal cord. A-C. αSD was identified by Western blot with PSer129 antibody (A), clone 42 (B) or LB509 (C) antibodies. In (D) the blots were revealed by an anti-β-actin antibody as a loading control. E-F. ELISA results in these same neuro-anatomical regions were obtained with PSer129 or LB509 antibodies, in mice inoculated with brain homogenates from sick M83 mice (P1) (n = 1, 4 repeats, mean ± SD) (E) or with fibrillar recombinant αS (n = 4, mean ± SD) (F). Molecular weight markers (in kDa) are indicated on the left of panels A-D. Equal amounts of total proteins were used for equivalent loading on gel. *corresponds to a non specific band as shown by the presence of a similar band in the absence of primary antibody and in αS-null mice (data not shown).