Intrathecal infection with VV causes lethal encephalitis. (A) C57BL/6 wild type mice were injected intrathecally by suboccipital puncture of the cisterna magna with various doses of VV. Percentage of surviving mice are depicted in Kaplan-Meier curves (n = 5 per group). (B) Virus loads in CNS and ovaries of wild type mice infected intrathecally with VV were measured on day 5 after infection (Student’s t test, n ≥ 5). (C) Wild type mice injected with VV were subjected to histologic analyses on day 5 after infection. Unless otherwise indicated, scale bars represent 200 μm in the low magnification and 20 μm in the higher magnification photomicrographs.