Illustrations of the substantia nigra (SN) and progressive supranuclear palsy (PSP) pathology. There is severe neuronal loss and gliosis (A) with neurofibrillary tangles (NFTs) (white arrow, B) in the SN. Tau immunohistochemistry demonstrates pre-tangles (PreT, black arrow, C) and occasional tufted astrocytes (TAs) in the motor cortex (D), and coiled bodies (CBs, arrowheads, E) in the posterior frontal cortical white matter, NFTs and neuropil threads (NTs) in the pontine base (F) and PreTs (black arrow, G), NFTs (white arrow, G) and threads in the dentate nucleus (G), CBs and fine NTs in the cerebellar white matter (H), and, TAs in the caudate nucleus (I, J). TAs in the motor cortex (F) and caudate (J) are stained positive using 4-repeat tau antibody but not 3-repeat tau antibody. A: H&E, B, C,-E, G-I: tau immunohistochemistry (AT8), F & J: 4-repeat tau immunohistochemistry. Bar in J = 50 μm in A-C, E, G, H = 25 μm in D, I, J and = 100 μm in F.