Figure 1

Schema of iCNA algorithm applied to paediatric high grade glioma. 1099 intragenic breakpoints were initially identified in a published series of 100 pHGG. From these, known copy number polymorphisms were excluded, as were these seen in a series of 26 matched normal DNA samples. After filtering for statistical significance and manual inspection of copy number plots, a series of 19 intragenic deletions, 18 amplicons, and 2 candidate fusions were identified.