Colocalization of MARK4, phospho-MARK, and p-tau Ser
in GVDs. Triple-immunofluorescence images from the CA1-field of an AD case (#32) with antibodies against pMARK (FITC), MARK4 (Cy3) and p-tau Ser262 (Cy5). (A) Arrowheads indicate neurons that stain positively for all three antibodies. (B) A typical pre-tangle neuron with perinuclear phospho-tau staining is magnified, where arrowheads indicate individual GVDs where all three markers colocalize. (C) A 3-D reconstruction of a Z-stack focused on the GVD in the bottom part of the neuron. Scale bar: 100 μm.