Figure 5

Western blot of MARK3 and MARK4 in hippocampal homogenates. (A) Western blots showing MARK3 (top) and MARK4 (bottom) immunoreactivities in hippocampal homogenates from 4 NDE and 3 AD cases. HEK cells transfected with either MARK3 or MARK4, respectively, served as positive controls, whereas non-transfected HEK cells served as negative control. β-actin served as a normalizing control for protein content in the homogenates. (B) Quantification of full-length (84 respective 82 kDa) and shorter (40–65 kDA) band densities normalized against β-actin band density. No full-length MARK4 band could be identified or measured. Data depicts means ± SEM. *p < 0.05, ns non-significant by Student’s unpaired two-tailed t-test.